Sample Submission Form ::

The following protocol describes a method to excise protein bands from 1-D and 2-D gels, stained with either Coomassie brilliant blue or silver, for identification by mass spectrometry analysis. Liquid samples are acceptable and should be kept frozen and shippped on dry ice.

Steps:

• Gloves must be worn at all times to minimize contamination from keratins and improve sample identification. If possible, proteins should be excised in a laminar flow hood.
  
  
• Prewash 1.5 mL microcentrifuge tubes that will be used for sample submission with 95% ethanol and air-dry prior to use.
  
  
• With a clean razor blade, excise the protein band or spot of interest only. For addional bands or spots the razor blade can be cleaned by washing repeatedly with methanol and water.
  
  
• Alternatively, excise three plugs per band using a syringe with blunt needle (1.7 mm ID) or blunted pipettor tip. Avoid capturing any surrounding non-stained polyacrylamide.
  
  
• Gel bands should be excised into 2 to 3 cubes.
  
  
• Place all pieces corresponding to the one band into a 1.5-mL microcentrifuge tube and add enough 1% acetic acid to cover the gel slice(s).
  
  
• Snap lid onto microcentrifuge tube firmly and wrap with parafilm.
  
  
• Store samples at -20°C until pick up or delivery.
  
  
• Complete On-line Sample Submission Form or download printable PDF HERE to complete and submit with samples.
  
  Our shipping address is:
  Custom Bioreseacrh
  2585 Meadowpine Blvd.
  Mississauga, ON
  L5N 8H9
  
  

ORDER INFORMATION:

Researcher:


Your Email:


Principal Investigator:


Address (Hospital/Institute/University) :


Dept/Floor/Room# :


Telelephone Number:


Fax Number:

BILLING INFORMATION:

Bill To :


Address:


Cost Ctr / P.O.# :

The following protocol describes a method to excise protein bands from 1-D and 2-D gels, stained with either Coomassie brilliant blue or silver, for identification by mass spectrometry analysis. Liquid samples are acceptable and should be kept frozen and shippped on dry ice.

Steps:

1. Gloves must be worn at all times to minimize contamination from keratins and improve sample identification. If possible, proteins should be excised in a laminar flow hood.
2. Prewash 1.5 mL microcentrifuge tubes that will be used for sample submission with 95% ethanol and air-dry prior to use.
3. With a clean razor blade, excise the protein band or spot of interest only. For addional bands or spots the razor blade can be cleaned by washing repeatedly with methanol and water.
4. Alternatively, excise three plugs per band using a syringe with blunt needle (1.7 mm ID) or blunted pipettor tip. Avoid capturing any surrounding non-stained polyacrylamide.
5. Gel bands should be excised into 2 to 3 cubes.
6. Place all pieces corresponding to the one band into a 1.5-mL microcentrifuge tube and add enough 1% acetic acid to cover the gel slice(s).
7. Snap lid onto microcentrifuge tube firmly and wrap with parafilm.
8. Store samples at -20°C until pick up or delivery.
9. Complete On-line Sample Submission Form or download printable PDF HERE to complete and submit with samples.
10. Our shipping address is:
    Custom Biologics
    1 Marmac Dr.
    Toronto, ON. Canada
    M9W 1E7

    .

ORDER INFORMATION:

Researcher:

Your Email:

Principal Investigator:

Address (Hospital/Institute/University) :

Dept/Floor/Room# :

Telelephone Number:

Fax Number:

BILLING INFORMATION:

Bill To :

Address:

Cost Ctr / P.O.# :

Your Sample ID   MW	Sample State     liquid, coomassie, silver

Service (Specify:)     MS, MS Plus, MS/MS	Enzyme Digest  (Trypsin or  specify if other)

Please fill in all applicable columns per submitted sampleComments / Special Instructions:

Samples are picked up every morning from 10am-12pm. Please call the day before to ensure your sample(s) are received in a timely manner. A separate report for each submitted sample will be sent to your email address within four (4) business days.


  CLICK HERE TO DOWNLOAD PRINTABLE PDF FORM

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